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KMID : 0545120140240040440
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Journal of Microbiology and Biotechnology
2014 Volume.24 No. 4 p.440 ~ p.446
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Improving Endoglucanase Activity by Adding the Carbohydrate-Binding Module from Corticium rolfsii
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Zizhong Tang
Hui Chen
Lijiao Chen
San Liu
Xueyi Han
Qi Wu
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Abstract
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The carbohydrate-binding module (CBM) is an important domain of most cellulases that plays a key role in the hydrolysis of cellulose. The neutral endoglucanase (EG1) gene was reconstructed. A redesigned endoglucanase, named EG2, was constructed with a CBM containing a linker from Corticium rolfsii (GenBank Accession No. D49448). The redesigned EG genes were expressed in Escherichia coli, and their characteristics are discussed. Results showed that the degradation of cellulose by EG2 was about double that by EG1. The specific activities of EG1 and EG2 were tested under optimal conditions, and EG2 had higher activity (169.1 ± 2.74 U/mg) toward CMC-Na than did EG1 (84.0 ± 1.98) in the process of cellulose degradation. The optimal pH and temperature, pH stability, and heat stability of EG1 and EG2 were similar. Results indicated that the CBM plays an essential role in the hydrolysis of cellulose. We can improve EG’s catalytic power by adding the CBM from Corticium rolfsii.
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KEYWORD
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Endoglucanase, Carbohydrate-binding module (CBM), Gene fusion, Enzyme properties, Corticium rolfsii
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